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Blood Collection Tubes and Additives

Collection Tubes and their Additives

Red Top
Contains:	None
Effects on Specimen:	Blood clots, and the serum is separated by centrifugation
Uses:	Chemistries, Immunology and Serology, Blood Bank (Crossmatch)

	Red-Gray Mottled Top ("Tiger top")
Contains:	Serum Separating Tube (SST) with clot activator
Effects on Specimen:	Forms clot quickly and separates the serum with SST gel at the bottom of the tube
Uses:	Blood type screening and chemistries

Gold Top
Contains:	Separating gel and clot activator
Effects on Specimen:	Serum separator tube (SST) contains a gel at the bottom to separate blood from serum on centrifugation
Uses:	Serology, endocrine, immunology, including HIV

Light Green Top
Contains:	Plasma Separating Tube (Na Heparin)
Effects on Specimen:	Anticoagulants with lithium heparin; Plasma is separated with PST gel at the bottom of the tube
Uses:	Chemistries

Lavender/Purple Top
Contains:	EDTA (liquid form)
Effects on Specimen:	Forms calcium salts to remove calcium
Uses:	Hematology (CBC) and Blood Bank (Crossmatch); requires full draw - invert 8 times to prevent clotting and platelet clumping

Light Blue Top
Contains:	Sodium citrate (Na Citrate)
Effects on Specimen:	Forms calcium salts to remove calcium
Uses:	Coagulation tests (PT, PTT, TCT, CMV), tube must be filled 100% full.

Dark Green Top
Contains:	Sodium heparin or lithium heparin
Effects on Specimen:	Inactivates thrombin and thromboplastin
Uses:	Ammonia, lactate, HLA typing For lithium level, use sodium heparin For ammonia level, use sodium or lithium heparin!

Dark Blue/Royal Blue Top
Contains:	Sodium heparin or Na2 EDTA
Effects on Specimen:	Forms calcium salts Tube is designed to contain no contaminating metals
Uses:	Toxicology and trace element testing (zinc, copper, lead, mercury) and drug level testing

Light Gray Top
Contains:	Sodium fluoride and potassium oxalate
Effects on Specimen:	Antiglycolytic agent preserves glucose up to 5 days
Uses:	For lithium level, use sodium heparin Glucoses, requires full draw (may cause hemolysis if short draw)

Yellow Top
Contains:	ACD (acid-citrate-dextrose)
Effects on Specimen:	Complement inactivation
Uses:	Paternity testing, DNA studies

Tan/Brown Top
Contains:	Sodium heparin
Effects on Specimen:	Inactivates thrombin and thromboplastin
Uses:	Serum lead determination

Black Top
Contains:	Sodium citrate (buffered)
Effects on Specimen:	Forms calcium salts to remove calcium
Uses:	Westergren Sedimentation Rate; requires full draw

Orange Top
Contains:	Thrombin
Effects on Specimen:	Quickly clots blood
Uses:	STAT serum chemistries

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