Venipuncture Technique Using the Vacutainer®
Drawing Blood with a Needle Holder System
Multisample Vacutainer® System Blood Collection
-- General Instructions:
Open needle package but do not remove needle shield - safety
needles should be used!
Thread needle onto holder. If drawing sterile sample, use sterile
Select tube or tubes appropriate for the type of sample
Tubes that contain additives should be gently tapped to dislodge any
additive which may be trapped around the stopper
OSHA's recent ban on tube holder reuse dictates use of a NEW tube
holder for every patient!
Insert tube into holder, then gently push tube so that the stopper is
penetrated by the needle
Select site for venipuncture by viewing and palpating all available
veins of both arms
Prepare venipuncture site with an appropriate antiseptic.
YOU MUST NOT PALPATE VENIPUNCTURE SITE AGAIN AFTER CLEANSING!
Secure patient's arm in a downward position that is stable but yet
comfortable for the patient
Remove needle shield
Push tube onto holder, puncturing diaphragm of stopper
Remove tourniquet as soon as blood appears in tube. Do not overfill
the collection tube
If no blood flows into tube or ceases to flow before an adequate
sample is collected, the following steps are suggested to complete satisfactory collection:
Confirm correct position of needle cannula in vein
If a multiple sample needle is being used, remove the tube and
place a new tube into the holder
If the second tube does not draw, remove needle and discard in
appropriate disposal device
Repeat procedure from Step 1
When first tube is adequately filled remove it from holder
Place succeeding tubes in holder (following correct order of draw),
puncturing diaphragm to initiate flow. Tubes without additives are drawn before tubes with
While each successive tube is filling, invert the previous tube
containing additives 8-10 times.
DO NOT SHAKE!
As soon as blood stops flowing in the last tube, remove needle from
Activate the needle shield's safety feature, and discard
If conventional needles are used do not recap needle, bend, or
shear. Dispose needle straight into proper container!
Apply pressure to puncture site with dry, sterile swab, until
Apply bandage if desired, except in infants and toddlers
Proper precautions should be taken to avoid contact with blood
droplets when handling tubes
Dispose of any holder that becomes contaminated with blood
A patient should be offered the option to return to the
office on a different day for another try after two failed attempts of finding the vein. Going to the
local hospital, a health care facility, or a freestanding reference laboratory where more experienced
phlebotomists are drawing blood could also be discussed as an option.
Trademark Notice: Vacutainer is a federally and internationally registered
trademark owned by BD (Becton, Dickinson and Company) that is used in connection with a complete
system of tubes, needles and needle holders, sharps collectors, and safety devices. Reference: Becton
Dickinson Trade Information # 109.